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Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases, and play an important role in the skeletal muscle damage through strenuous exercise or its aging. To investigate whether H₂O₂, as main precursor of highly reactive free radicals, induces apoptosis in mouse skeletal muscle cells C2C12, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide(MTT) assay, terminal deoxynuclotidyl transferase -mediated dUTP nick end-labeling (TUNEL) assay, 4,6-diamidino-2- phenylindole (DAPI) staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were performed. Through morphological and biochemical analyses, it was demonstrated that C2C12 cells treated with H₂O₂ exhibit classical apoptotic features. In addition, it was shown that H₂O₂ induces increases in the levels of bax, and a decrease in the bcl-2 expression. Based on the results, H₂O₂ appears to activate specific intracellular death-related pathways and the induction of apoptosis in skeletal muscle cells.
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